FIG. 4.

Abnormal chemotaxis of ILK-deficient T cells. Chemotaxis assays were performed on thymocytes from 3- to 4-week-old mice in transwell chambers to the lymphocyte chemokines (a) CCL19 (n = 4, *P < 0.01) and (b) CXCL12 (n = 7, *P < 0.01). (c) CXCL12-triggered chemotaxis of purified lymph node B cells from the same animals (n = 4, differences not significant). Cumulative data from the indicated number of experiments are expressed as the means ± standard errors of the means. (d) Representative flow-cytometric analysis of surface CXCR₄ expression on T cells. fl/fl, flox/flox. (e) Chemotaxis assays to CXCL12 were performed on flow-sorted lymph node T cells from YFP indicator⁺ Lck-Cre⁺/ILK^flox/flox and YFP indicator⁺ Lck-Cre⁺/ILK^WT/WT mice. Cumulative data from three experiments are shown. YFP⁻ T cells were only studied at 10 nM CXCL12 due to restricted yields. *, P < 0.01, compared to YFP indicator⁺ Lck-Cre⁺/ILK^WT/WT T cells; **, P < 0.01 compared to YFP indicator⁺ Lck-Cre⁺/ILK^flox/flox T cells; p = NS, differences not significant.