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. 2005 Dec;25(24):10721–10730. doi: 10.1128/MCB.25.24.10721-10730.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 2. — The crb2-T215A mutation decreases IR-induced Crb2 phosphorylation but not focus formation. (A) Immunoblot analysis of Crb2 in asynchronous cultures of cells harvested immediately after irradiation with the indicated dose of IR. The position of hyperphosphorylated Crb2 is indicated by the asterisk. The immunoblot was quantified using ImageJ software. (B) YFP-Crb2 localization was observed for indicated cells harvested immediately after irradiation with IR (36 Gy). Size bar, 5 μm. (C) Time-dependent changes in YFP-Crb2 foci after exposure to IR (36 Gy). Numbers of cells with Crb2 foci at various times after IR treatment were determined and plotted as percentages of cells with Crb2 foci.