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Cdc2 activity is required for efficient phosphorylation of Chk1 in hta1,2-AQE cells. Inactivation of Cdc2 in the hta1,2-AQE background specifically reduces DNA damage-induced Chk1 mobility shift in response to 5 mU/ml bleomycin treatment for 40 min. Cells were treated at 25°C or 36°C as indicated. The Chk1 mobility change caused by phosphorylation was quantified as described in the legend to Fig. 1.
