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Effect of dsbC and trbB null mutations on pOX38-Tc transfer
| Construct | No. of transconjugants | Mating efficiency (±10) (no. of transconjugants/100 donor cells) |
|---|---|---|
| pOX38-Tc/RI89 (wild type) | 1.2 × 108 | 80 |
| pOX38-Tc/RI179 (dsbC) | 1.1 × 108 | 73 |
| pOX38-TcΔtrbB::cat/RI89 (wild type) | 1.0 × 108 | 67 |
| pOX38-TcΔtrbB::cat/RI179 (dsbC) | 1.1 × 107 | 7 |
| pOX38-TcΔtrbB::cat/RI179 (dsbC) + pFTrbB | 1.0 × 108 | 67 |
| pOX38-TcΔtrbB::cat/RI179 (dsbC) + pBAD30 | 1.0 × 107 | 7 |
