TABLE 5.
Effect of overproduction of F- and H-like transfer proteins in a dsbA mutanta
| E. coli strain | DTT concn (mM) | Plasmid construct | No. of transconjugants |
|---|---|---|---|
| pOX38-Tc/RI89 (wild type) | 1.2 × 108 | ||
| pOX38-Tc/RI90 (dsbA) | 1.1 × 104 | ||
| pOX38-Tc/RI90 (dsbA) | 1 | <10 | |
| pOX38-Tc/RI90 (dsbA) | 1 | pBAD30 | <10 |
| pOX38-Tc/RI90 (dsbA) | 1 | pFTraFb | <10 |
| pOX38-Tc/RI90 (dsbA) | 1 | pFTraF(CPYC)b | <10 |
| pOX38-Tc/RI90 (dsbA) | 1 | pFTrbBb | 9.0 × 103 |
| pOX38-Tc/RI90 (dsbA) | 1 | pFTrbB(Y63H)b | 3.0 × 103 |
| pOX38-Tc/RI90 (dsbA) | 1 | pFTrbB(SPYS)b | <10 |
| pOX38-Tc/RI90 (dsbA) | 1 | pHTrhFc | <10 |
| pOX38-Tc/RI90 (dsbA) | 1 | pHHtdTa | 8.0 × 103 |
| pOX38-Tc/RI90 (dsbA) | 1 | pHDsbCc | 1.0 × 104 |
| pOX38-Tc/RI90 (dsbA) | 1 | pHDsbC(SGFS)c | <10 |
a
All vector constructs were induced with the addition of 0.1% arabinose 2 hours prior to adding the recipient cells for the mating assay.
b
F-like.
c
H-like.