TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and property | Source or reference |
|---|---|---|
| E. coli strains | ||
| W3110 | Wild type | E. coli Stock Center |
| LW12 | W3110 ΔluxS::Kan | This study |
| ZK126 | W3110 ΔlacU169 tna-2 | 9 |
| ZK1000 | ZK126 ΔrpoS::Kan | 2 |
| LW2 | ZK126 Δcrp::Kan | 50 |
| LW7 | ZK126 ΔluxS::Kan | 50 |
| LW8 | ZK126 ΔlsrR::Kan | 50 |
| LW9 | ZK126 Δ(lsrACDBFG)::Kan | 50 |
| LW11 | ZK126 ΔlsrK::Kan | 50 |
| Plasmids | ||
| pFZY1 | galK′-lacZYA transcriptional fusion vector, Apr | 21 |
| pJLlsrR | pFZY1 derivative, containing lsrR promoter region, Apr | This study |
| pJLlsrK | pFZY1 derivative, containing lsrK promoter region, Apr | This study |