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. 2005 Dec;187(24):8504–8506. doi: 10.1128/JB.187.24.8504-8506.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 2. — Gel shift assay. (A) The dofA promoter. The probe containing the region from nt −150 to −32 was mixed with FruA-DBD-His₈, and the binding patterns were analyzed by PAGE. Lane 1, no FruA-DBD-His₈; lane 2, 0.5 ng/μl of FruA-DBD-His₈; lane 3, 1 ng/μl of FruA-DBD-His₈; lane 4, 1 ng/μl of FruA-DBD-His₈ and anti-FruA antibody. FruA-DBD-His₈/dofA promoter complexes are indicated by arrows. Anti-FruA antibody/FruA-DBD-His₈/dofA promoter complexes are indicated by an arrowhead. (B) The tps promoter. The probe contains the region from nt −250 to −41. Lane 1, no FruA-DBD-His₈; lane 2, 0.5 ng/μl of FruA-DBD-His₈; lane 3, 1 ng/μl of FruA-DBD-His₈.