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Stability of alginate production in Pf20118algLH203R. Pf20118 (dark gray bars), Pf20118algLH203R (white bars), and Pf20118algLH203R::TnKB16 (light gray bars) were grown in liquid cultures (shake flasks) and reinoculated in fresh medium (2%) every second day. The percentage of mucoid colonies was determined by plating of samples of each culture on agar medium (PIA). The error bars indicate the standard deviations of three parallel cultures.
