FIG. 5.

RPA to analyze mRNA levels of SP1176 (ptsI) (A) and SP1128 (enolase) (B) in wild-type (Wt) strain AC353 and ccpA strain RI1932. Riboprobes for SP1176 (ptsI), SP1128 (enolase), and SP1414 (rpsU loading control) were generated and hybridized to 3 μg of RNA from the three S. pneumoniae strains. RNA was harvested from cells grown in THB to an OD₆₀₀ of 0.3. Undigested riboprobes of SP1176, SP1414, and SP1128 in the absence of S. pneumoniae RNA are indicated. An rpoB riboprobe is also indicated but was not used in the experiments. The value below a lane is the relative abundance of the SP1176 or SP1128 transcript in the wild-type or ccpA strain normalized to SP1414 (rpsU), as calculated by densitometry with ImageQuant TL software (Amersham Biosciences).