TABLE 2.
Sequences of primers used in this study
| Primer | Contig no.; coordinates or source | Sequencea (5′ to 3′) |
|---|---|---|
| ccpupF | AE007488; 11641-11660 | GCTGCCAAGTATGTCACCAA |
| ccpupR | AE007489; 358-339 | GAAGAAGGTTTTTATATTACAGCTCCAAAAAAATCAGGGAATCGAGA |
| ccpdnF | AE007489; 1370-1390 | CATCAAGCTTATCGATACCGTCCTTTTCCTGTCCTTTCTAT |
| ccpdnR | AE007489; 2390-2368 | AAAGTATTAGTCGCAGAAGATCA |
| ccpF | AE007489; 487-504 | GAGGTTGGGCAATCGTTG |
| ccpR | AE007489; 1069-1049 | AATAGCGATGAAGATAACGAG |
| ccpF0 | AE007488; 11593-11610 | AGGGCTGCTGACAAAGGA |
| ccpR0 | AE007489; 2430-2411 | AGTGCAGGTTCGACTACTTT |
| catF1 | pAC1000 | CGGTATCGATAAGCTTGATG |
| NcatR1 | pAC1000 | TGGAGCTGTAATATAAAAACCTTC |
| lacEF1 | AE007358; 10181-10200 | TTTAGAGGCTCCTATTTTTT |
| lacER1 | AE007359; 96-114 | CACCGGAACTCCTTTTTTT |
| lacER2 | AE007359; 7860-7841 | CCTATCTGGTCAGTATCGGA |
| lacESp | AE007359; 114-96 | AAAAAAAGGAGTTCCGGTGCCCAGATCTACCGCTCTAGAACTAGTGGATCCC |
| SpcR1 | pR412 | CCCAGATCTCAATTTTTTTATAATTTTTTTAATCTG |
| SpcF1 | pR412 | CCCAGATCTACCGCTCTAGAACTAGTGGATCCC |
| Ccp1 | AE007489; 1390-1370 | ATAGAAAGGACAGGAAAAGGA |
| Ccp2 | AE007489; 339-358 | TCTCGATTCCCTGATTTTTT |
| Spccp1 | AE007489; 1390-1370 | CAGATTAAAAAAATTATAAAAAAATTGAGATCTGGGATAGAAAGGACAGGAAAAGGA |
| CplacE | AE007489; 358-339 | AAAAAATCAGGGAATCGAGAGCTGTGTAGTAAGTTTTTCCA |
| NlacEF | AE007358; 10261-10283 | GGGTATTGTGTGGATTAAAAAGG |
| NlacER | AE007359; 7736-7713 | ACTGGTTTCTACAGGCTTGATTAG |
| 76rpa | AE007418; 8260-8277 | TAATACGACTCACTATAGGGAGATCACCACACATACCAGCC |
| Sal76 | AE007418; 8051-8071 | AGCGTCGACTCAATCAAAATTAACGTATTCTT |
| 28rpa | AE007414; 1192-1173 | TAATACGACTCACTATAGGGAGAGCCATTTCGATAGCTTCAA |
| Sal128 | AE007414; 1417-1396 | AGCGTCGACTTATTTTTTAAGGTTGTAGAAT |
| 1414F | AE007438; 6294-6276 | ATGTCTAAAACAGTAGTAC |
| 1414R | AE007438; 6118-6140 | TAATACGACTCACTATAGGGAGTTAGAATTTTTTACGTTTACGAG |
a
Underlined sequences are complementary to the S. pneumoniae genomic DNA. Coordinates are provided in the middle column.