FIG. 3.

Transcriptional analysis of the B. breve UCC 2003 clpP operon. (a) Slot blot hybridization using RNAs extracted from cells incubated for up to 150 min at various temperatures or with various NaCl concentrations (indicated in the left-hand margin). (b) Schematic representation of mRNA levels of induction. The different colors and filling of the pillars correspond to the various times for which heat and osmotic shocks were applied, as indicated in the figure. (c) Position of transcript with respect to the clpP locus map. The estimated size of the transcript is indicated. Hairpin symbols indicate possible rho-independent terminators. (d) Northern blot analysis of the B. breve UCC 2003 clpP operon performed using clpP1 as a probe and total mRNA isolated from cultures exposed to 37°C or under heat or hyperosmotic conditions for the times indicated.