TABLE 3.
Production of specific cytokines by bone marrow-derived dendritic cells after in vitro stimulation with specific antigens
| Stimulant | Concn of cytokine secreted by dendritic cells (pg/ml)a
|
|||
|---|---|---|---|---|
| IL-12 p40 | IL-12 p70 | IL-10 | TNF-α | |
| Medium | —b | — | 41.9 ± 0.1 | — |
| E. coli LPS | 400.7 ± 2.9 | 451.4 ± 65.8 | 1,255.7 ± 7.8 | 388.2 ± 43.8 |
| Irradiated RB51G/LacZ | 580.1 ± 79.3* | 481.7 ± 10.2** | 126.6 ± 3.9 | — |
| Heat-killed RB51G/LacZ | 329.5 ± 39.9* | 201.8 ± 16.3** | 119.1 ± 2.6 | 24.0 ± 0.6 |
a
Values are means ± standard deviations. There were significant differences between means with the same number of asterisks by Student's t test (P < 0.05).
b
—, below the level of detection.