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Based on 47 ELISA plates tested between 27 January and 16 March 2002 for anti-K39 antibodies, the OD mean reading at 450 nm for 11 negative controls (9 from a nonendemic area in Bangladesh and 2 nonexposed North Americans) varied from 0.059 to 0.880 (median, 0.196; mean, 0.230) (A). The standard deviation was also inconsistent (range, 0.0024 to 0.0423; median, 0.013; mean, 0.015) (B). Consequently, these deviations resulted in variable cutoff points.
