Confirmation of rat Golfalpha PCR product using BamHI restriction endonuclease digestion. Since there was a single BamHI site within the 445 base pair (bp) amplicon, two fragments were expected to result after digestion, one 241 bp and the other 204 bp. These can be seen in lanes 2 and 3 for the vestibular end organs and Scarpa's ganglia, respectively. A 50-1,000 bp DNA ladder is shown in lane 1 (Cambrex, East Rutherford, NJ).