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New species-specific RLB probes used in this study
| Oligonucleotide probe specificity | 18S rRNA sequence (5′→3′) | GenBank accession no(s). | Tm (°C)a |
|---|---|---|---|
| Theileria sp. (greater kudu) | CTG CAT TGT TTC TTT CCT TTG | AY748465 | 54.0 |
| Theileria sp. (grey duiker) | CAT TTT GGT TAT TGC ATT GTG G | AY748466 | 56.3 |
| Theileria spp. (sable) | GCT GCA TTG CCT TTT CTC C | L19081, AY748462 to AY748464 | 56.7 |
The melting point (Tm) was determined empirically.
