TABLE 4.
Cytotoxicity analysis of heat-treated toxin preparations from Bacillus cereus
| Bacillus cereus toxin preparation (treatment) | % Cytotoxicity (avg ± SD)a |
|---|---|
| F 4810b (none) | 66.56 ± 0.11A |
| F 4810 (58°C, 40 min) | 0.75 ± 0.01D |
| HS23-11 (none) | 45.59 ± 0.15B |
| HS23-11 (58°C, 40 min) | 0.94 ± 0.02D |
| CA1c (none) | 4.13 ± 0.04D |
| CA1 (58°C, 40 min) | 2.46 ± 0.07D |
| A926 (none) | 51.56 ± 2.35B |
| A926 (58°C, 40 min) | 8.20 ± 1.02C |
| A926 (70°C, 15 min) | 2.48 ± 0.05D |
| ATCC 14579 (none) | 63.89 ± 3.01A |
| ATCC 14579 (58°C, 40 min) | 11.04 ± 5.58C |
| ATTC 14579 (70°C, 15 min) | 5.06 ± 0.04D |
a
Data are averages from two experiments analyzed in duplicate. Values followed by different superscript letters (A through D) are significantly different (P< 0.05). In this experiment, the cytotoxicity value for the negative-control strain (B. subtilis) was about 2.24%.
b
Emetic-toxin-producing strain.
c
Considered a noncytotoxic strain (see Table 2).