TABLE 5.

Nucleotide sequences of specific primers and product sizes for toxin genes of B. cereus

Toxin gene	Primer sequence (5′-3′)	PCR conditionsa	Product size (bp)	Reference
hblA	GCTAATCTAGTTTCACCTGTAGCAAC	35 cycles of 94°C for 20 s, 58°C for 40 s, and 72°C for 40 s	874	25
	AATCATGCCACTGCGTGGACATATAA
bceT	TTACATTACCAGGACGTGCTT	30 cycles of 94°C for 15 s, 55°C for 45 s, and 72°C for 2 min	428	1
	TGTTTGTGATTGTAATTCAGG
cytK	AACAGATATCGGTCAAAATGC	30 cycles of 94°C for 1 min, 52°C for 1 min, and 72°C for 1 min	623	32
	CGTGCATCTGTTTCATGAGG
nheA	TACGCTAAGGAGGGGCA	Multiplex reaction	499	20
	GTTTTTATTGCTTCATCGGCT
nheB	CTATCAGCACTTATGGCAG	30 cycles of 94°C for 15 s, 55°C for 45 s, and 72°C for 2 min	769
	ACTCCTAGCGGTGTTCC
nheC	CGGTAGTGATTGCTGGG		581
	CAGCATTCGTACTTGCCAA

^aPreceded by initial denaturation at 94°C for 5 min and followed by a final extension at 72°C for 7 min.