TABLE 1.
Forward and reverse sequences of primersc
| Gene (function) | Primer sequence (5′-3′)a | Annealing temp (°C) | Amplicon size (bp)b |
|---|---|---|---|
| adk (adenylate kinase) | ATTCTGCTTGGCGCTCCGGG (F) | 52 | 583 |
| CCGTCAACTTTCGCGTATTT (R) | |||
| fumC (fumarate hydratase) | TCACAGGTCGCCAGCGCTTC (F) | 52 | 806 |
| GTACGCAGCGAAAAAGATTC (R) | |||
| icd (isocitrate/isopropylmalate dehydrogenase) | ATGGAAAGTAAAGTAGTTGTTCCGGCACA (F) | 52 | 878 |
| GGACGCAGCAGGATCTGTT (R) | |||
| purA (adenylosuccinate dehydrogenase) | CGCGCTGATGAAAGAGATGA (F) | 54 | 816 |
| CATACGGTAAGCCACGCAGA (R) | |||
| gyrB (DNA gyrase) | TCGGCGACACGGATGACGGC (F) | 58 | 911 |
| ATCAGGCCTTCACGCGCATC (R) | |||
| recA (ATP/GTP binding motif) | CGCATTCGCTTTACCCTGACC (F) | 58 | 780 |
| TCGTCGAAATCTACGGACCGGA (R) | |||
| mdh (malate dehydrogenase) | ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG (F) | 58 | 932 |
| TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT (R) |
a
F, forward; R, reverse.
b
The sequenced DNA strands were shorter than the original PCR amplicon.
c
Primers are maintained at http://web.mpiib-berlin.mpg.de and http://www.mlst.net.