TABLE 7.

Immunoreactivity of recombinant antigens with IgG subtype antibodies in serum samples from infants with congenital T. gondii infection^a

Patient no.	IgG level (IU/ml)	Time after birth (wk)	MIC2a Rec-ELISA cutoff				SAG1 Rec-ELISA cutoff
			IgG1	IgG2	IgG3	IgG4	IgG1	IgG2	IgG3	IgG4
T1	905	7	23.5	1.0	2.5	1.0	7.7	1.2	0.5	0.7
	220	13	10.4	1.0	1.3	0.8	4.9	1.5	0.5	0.7
T2	300	1	4.3	1.2	1.1	0.8	2.9	4.3	1.6	0.9
	275	4	2.9	0.7	0.7	0.7	1.8	1.5	0.9	0.6
T4	153	1	3.6	2.3	1.5	1.0	1.1	0.7	0.4	0.5
	87	5	2.1	2.6	1.4	1.0	1.3	2.4	1.2	0.8
T5	277	6	2.3	0.8	0.7	0.8	1.0	1.3	0.7	0.7
	146	10	1.5	0.8	0.6	0.9	0.9	1.5	0.6	0.6
T7	240	7	2.6	2.0	0.8	0.9	1.4	0.7	0.8	0.6
	190	11	2.6	1.5	1.2	0.8	2.0	1.3	1.0	0.5
T8	206	2	2.3	0.9	0.8	0.8	2.0	1.6	0.6	0.9
	64	8	1.4	0.8	0.7	0.9	1.2	2.8	0.6	1.0
T9	124	2	4.0	2.5	1.3	1.0	2.7	2.6	1.8	1.5
	73	7	2.8	4.1	1.7	1.2	1.4	4.4	1.3	1.0
T10	226	2	3.9	1.2	1.1	1.0	2.4	1.1	0.9	0.5
	216	4	2.3	1.7	1.8	0.7	1.9	0.7	0.8	0.5
T11	157	2	0.9	0.8	1.2	0.7	0.9	1.1	1.0	0.8
	140	7	3.0	0.9	0.9	0.7	1.2	1.3	0.7	0.6
T12	148	3	2.7	1.3	1.1	0.7	1.1	1.5	1.2	0.6
	136	6	2.0	1.9	1.2	0.9	1.1	2.0	1.5	0.6
T13	57	3	0.9	0.9	1.2	0.8	0.8	1.3	1.1	0.6
	39	8	0.8	1.2	0.8	0.8	0.7	1.2	0.6	0.6
T14	73	3	1.3	1.0	0.9	0.7	0.7	0.6	0.3	0.6
	43	9	0.9	0.9	0.8	0.7	0.7	0.5	0.4	0.5
T20	153	4	2.6	0.8	0.8	0.5	1.1	0.7	0.6	0.5
	132	8	1.9	0.9	0.8	0.6	0.9	0.7	0.5	0.6
T22	218	4	2.0	0.9	0.8	0.7	1.9	1.3	0.9	0.8
	221	6	1.6	0.9	0.7	0.7	1.3	1.1	0.8	0.8
T24	47	5	0.9	1.1	0.6	0.8	1.3	2.0	0.5	0.7
	30	9	0.9	0.9	0.6	0.8	0.8	2.3	0.5	0.6
T25	1,147	5	11.8	1.2	2.7	0.6	2.3	1.2	0.7	0.7
		17	1.2	0.9	1.0	0.8	0.8	0.9	0.7	0.5
T29	292	6	25.2	1.3	5.8	0.7	2.9	0.8	1.1	0.6
	300	10	14.6	1.1	3.8	0.5	2.8	0.7	0.8	0.5
T30	39	3	2.2	0.9	1.3	0.7	0.8	1.0	0.4	0.5
	28	6	9.7	1.0	8.3	0.7	0.8	0.9	0.3	0.5
T34	59	8	3.3	0.7	0.9	0.9	1.1	1.1	0.4	0.8
	176	13	6.9	0.9	3.1	1.0	1.0	1.6	0.6	0.6
T35	91	4	1.3	0.9	0.8	0.5	1.3	1.7	1.0	0.4
	67	8	0.9	0.8	0.4	0.4	1.0	0.9	0.5	0.5

^aSerial serum samples from infants with congenital toxoplasmosis were analyzed using IgG subtype Rec-ELISAs with GST fusion products. The values were calculated as the ratio of the test OD to the cutoff of the corresponding antigen. Italic type, OD > cutoff; bold type, serum sample pairs showing increasing IgG subtype reactivity with time.