TABLE 1.
Clinical materials, patient characteristics, and methods of detectiona
| Sampleb | Syndrome | Patient sample | Patient age | Epidemiology | Cell culture | Antigen detectionc
|
|
|---|---|---|---|---|---|---|---|
| IFA | LA | ||||||
| SO3360_01 | Bronchiolitis | NPA | 1.6 yr | Sporadic | cont | − | ND |
| SO3790_03 | Bronchiolitis | NPA | <5 yr | Sporadic | + | + | ND |
| SO4010_04 | Bronchiolitis | NPA | 1.1 yr | Sporadic | − | − | ND |
| SO4363_04 | Bronchiolitis | NPA | 10 mo | Sporadic | ND | − | ND |
| SO4366_04 | Bronchiolitis | NPA | 11 mo | Sporadic | ND | − | ND |
| SO4390_04 | Bronchiolitis | NPA | <5 yr | Sporadic | cont | + | ND |
| SO4405_04 | Bronchiolitis | NPA | <5 yr | Sporadic | + | + | ND |
| SO4408_04 | Bronchiolitis | NPA | <5 yr | Sporadic | ND | − | ND |
| SO4425_04 | Bronchiolitis | NPA | 1.5 yr | Sporadic | + | + | ND |
| SO4427_04 | Bronchiolitis | NPA | <5 yr | Sporadic | + | + | ND |
| SO4429_04 | Bronchiolitis | NPA | <5 yr | Sporadic | − | − | ND |
| G1066_01 | Influenza-like | TS | 33 yr | Sporadic | − | − | ND |
| G1108_01 | Influenza-like | TS | 26 yr | Sporadic | − | − | ND |
| G1253_02 | Influenza-like | TS | 2 yr | Sporadic | − | − | ND |
| G1507_04 | Influenza-like | TS | 33 yr | Sporadic | − | ND | ND |
| G1508_04 | Influenza-like | TS | 25 yr | Sporadic | − | − | ND |
| G1634_04 | Influenza-like | TS | 29 yr | Sporadic | − | − | ND |
| R1625_04 | Pneumonia | NW | 20 d | Sporadic | ND | ND | ND |
| 1184X_93 | Pneumonia | BAL | <5 yr | Sporadic | + | ND | ND |
| R1629_04 | HIV+, pneumonia | BAL | 30 yr | Sporadic | − | ND | ND |
| 591Fi_01 | LRI | BAL | Sporadic | − | ND | ND | |
| 992Fi_01 | LRI | BAL | Sporadic | − | ND | ND | |
| 593Fi_01 | LRI | BAL | Sporadic | − | ND | ND | |
| 130C_99 | LRI | NW | 19 mo | Sporadic | ND | ND | ND |
| R1612_04 | BMTx, hemorrhagic cystitis | NW | 14 yr | Sporadic | − | ND | ND |
| 226l_03 | BMTx, hemorrhagic cystitis | Urine | 40 yr | Sporadic | ND | ND | ND |
| 1938l_89 | BMTx, hemorrhagic cystitis | Urine | 30 yr | Sporadic | + | ND | ND |
| 1370l_03 | BMTx, hemorrhagic cystitis | Urine | 15 yr | Sporadic | ND | ND | ND |
| 411I_04 | LITx, fatal hepatitis | Liver | 5 yr | Sporadic | ND | ND | ND |
| 1803F_03 | Exanthema, fever | TS | 3 yr | Sporadic | ND | ND | ND |
| 2549F_03 | Exanthema, fever | TS | 4 yr | Sporadic | ND | ND | ND |
| 1804F_03 | Exanthema, fever | TS | 6 yr | Sporadic | ND | ND | ND |
| 1292F_01 | Exanthema, not fever | TS | 2 yr | Sporadic | + | ND | ND |
| 824O_02 | Keratoconjunctivitis | Eye swab | 56 yr | Sporadic | ND | ND | ND |
| 928O_04 | Keratoconjunctivitis | Eye swab | 30 yr | Out-Jaén | ND | ND | ND |
| 926O_04 | Keratoconjunctivitis | Eye swab | 55 yr | Out-Jaén | ND | ND | ND |
| 925O_04 | Keratoconjunctivitis | Eye swab | 61 yr | Out-Jaén | ND | ND | ND |
| D0005_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0007_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0001_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0011_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0002_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0004_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0015_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0016_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| D0017_01 | Diarrhea | Feces | <14 yr | Sporadic | − | ND | + |
| R1650_04 | BMTx, diarrhea | Feces | <5 yr | Sporadic | Hep-2 | + | ND |
| R1641_04 | BMTx, diarrhea | Feces | <5 yr | Sporadic | Hep-2 | + | ND |
| C1640_03 | BMTx, diarrhea | Feces | 6 yr | Sporadic | HEF | + | ND |
| R1647_04 | BMTx, diarrhea | Feces | <5 yr | Sporadic | Hep-2 | + | ND |
| C5335_01 | BMTx, pneumonia | BAL + biopsy | 49 yr | Sporadic | HEF | + | ND |
| 594_89 | HIV+, diarrhea | Feces | >40 yr | Sporadic | Hep-2 | + | ND |
| 4030_96 | Influenza-like | Feces | 3 yr | Sporadic | Hep-2 | + | ND |
| C1662_03 | Bronchiolitis | NPA | 5 mo | Sporadic | HEF | + | ND |
| C1491_02 | Bronchiolitis | NPA | 1 mo | Sporadic | HEF | + | ND |
| C1519_02 | Bronchiolitis | NPA | 15 mo | Sporadic | HEF | + | ND |
| C1201_00 | Bronchiolitis | NPA | 8 mo | Sporadic | HEF | + | ND |
| G3093_03 | LRI | Feces | 30 yr | Sporadic | Hep-2 | + | ND |
| C1167_00 | LRI | NPA | 3 mo | Sporadic | HEF | + | ND |
| 859_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Tenerife | Hep-2 | + | ND |
| 860_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Tenerife | Hep-2 | + | ND |
| 519_93 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Toledo | Hep-2 | + | ND |
| 856_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Tenerife | Hep-2 | + | ND |
| 615_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Pamplona | Hep-2 | + | ND |
| 636_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Pamplona | Hep-2 | + | ND |
| 647_96 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Pamplona | Hep-2 | + | ND |
| 841_94 | Keratoconjunctivitis | Eye swab | >40 yr | Sporadic | Hep-2 | + | ND |
| 43024_02 | Keratoconjunctivitis | TS | >40 yr | Out-Madrid | HEF | + | ND |
| C4292_02 | Keratoconjunctivitis | Eye swab | >40 yr | Out-Madrid | HEF | + | ND |
| C1629_02 | Newborn control | NPA | 0 d | Sporadic | HEF | + | ND |
| G1T4_03 | Environmental water | A549 | + | ND | |||
Abbreviations: BMTx, bone marrow transplant; LITx, liver and intestinal transplant; LRI, low respiratory infection; NPA, nasopharyngeal aspirates; TS, throat swab; BAL, bronchoalveolar lavage; NW, nasal wash; LA, latex agglutination; cont, contamination of cell cultures; ND, not done; Out-, outbreak; HEF, human embryonic fibroblast cell line.
Clinical sample identification no._year or isolate_year (boldface).
Cells were collected and stained by standard methods. The monoclonal antibodies used for detection of the fusion protein (F0/F1) of all strains of parainfluenza virus type 1, hemagglutinin of all strains of parainfluenza viruses types 2 and 3, nucleoproteins of influenza A and B, hemagglutinins H1 and H3 of influenza A, and fusion protein and nuclear protein of respiratory syncytial virus strains and enteroviruses were obtained from Chemicon. The immunofluorescence assay was performed with fluorescein isothiocyanate-conjugated goat antimouse immunoglobulin G (Sigma). All specimens for culture were collected in 3 ml of virus transport medium (MEM, Gibco-BRL, Life Technologies, Paisley, Scotland; penicillin, 200 U/ml; and streptomycin, 200 mg/ml; BioWhittaker, MA; mycostatin, 200 U/ml, Sigma; bovine serum albumin, 0.25%, Merck, Darmstadt, Germany). Stool samples were tested for HAdV (HAdV-40 and -41) by latex agglutination (Adenolex, Orion, Helsinki, Finland).