TABLE 2.
Sequence analysis of clinical samples and HAdV isolates
| Name | Speciesa | Typing by phylogeny
|
Typing by pairwise alignment
|
||
|---|---|---|---|---|---|
| Bootstrap value | Serotypeb | NW homology score (%)c | Serotype | ||
| Clinical samples | |||||
| G1066_01 | C | 79 | HAdV-2 | 98.5 | HAdV-2 |
| SO4010_04 | C | 79 | HAdV-2 | 100.0 | HAdV-2 |
| G1634_04 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| R1625_04 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| SO4425_04 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| SO4429_04 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| G1108_01 | E | 99 | HAdV-4 | 93.1 | HAdV-4 |
| 1292F_01 | E | 99 | HAdV-4 | 95.6 | HAdV-4 |
| 1803F_03 | E | 99 | HAdV-4 | 93.1 | HAdV-4 |
| SO3360_01 | C | 99 | HAdV-5 | 96.1 | HAdV-5 |
| SO3790_03 | C | 99 | HAdV-5 | 100.0 | HAdV-5 |
| SO4366_04 | C | 99 | HAdV-5 | 96.1 | HAdV-5 |
| 591F_01 | C | 99 | HAdV-5 | 99.5 | HAdV-5 |
| 992F_01 | C | 99 | HAdV-5 | 99.5 | HAdV-5 |
| 130C_99 | C | 99 | HAdV-5 | 99.5 | HAdV-5 |
| 2549F_03 | C | 99 | HAdV-5 | 99.0 | HAdV-5 |
| 1804F_03 | C | 99 | HAdV-5 | 98.5 | HAdV-5 |
| G1507_04 | C | 59 | HAdV-6 | 99.0 | HAdV-6 |
| SO4408_04 | C | 59 | HAdV-6 | 99.5 | HAdV-6 |
| SO4427_04 | C | 59 | HAdV-6 | 99.5 | HAdV-6 |
| G1508_04 | C | 59 | HAdV-6 | 99.0 | HAdV-6 |
| 593F_01 | C | 59 | HAdV-6 | 98.5 | HAdV-6 |
| 1370l_03 | C | 59 | HAdV-6 | 99.5 | HAdV-6 |
| 411l_04 | C | 59 | HAdV-6 | 99.5 | HAdV-6 |
| D0016_01 | C | 59 | HAdV-6 | 99.5 | HAdV-6 |
| SO4363_04 | C | 59 | HAdV-6 | 99.1 | HAdV-6 |
| SO4405_04 | B | 98 | HAdV-7 genotype 2 | 99.5 | HAdV-7 genotype 2 |
| G1253_02 | B | 98 | HAdV-7 genotype 2 | 99.5 | HAdV-7 genotype 2 |
| 1184X_93 | B | 98 | HAdV-7 genotype 2 | 99.5 | HAdV-7 genotype 2 |
| 824O_02 | B | 98 | HAdV-7 genotype 2 | 99.0 | HAdV-7 genotype 2 |
| R1629_04 | B | 61 | HAdV-11, 34 or 35 | 98.2* | HADV-11, 34 or 35 |
| R1612_04 | B | 61 | HAdV-11, 34 or 35 | 97.8* | HADV-11, 34 or 35 |
| 1938l_89 | B | 61 | HAdV-11, 34 or 35 | 99.2* | HADV-11, 34 or 35 |
| 226l_03 | B | 71 | HAdV-14 | 96.6 | HAdV-14 |
| D0002_01 | F | 99 | HAdV-40 | 100.0 | HAdV-40 |
| D0004_01 | F | 99 | HAdV-40 | 98.5 | HAdV-40 |
| D0017_01 | F | 99 | HAdV-40 | 100.0 | HAdV-40 |
| D0015_01 | F | 99 | HAdV-40 | 100.0 | HAdV-40 |
| D0005_01 | F | 99 | HAdV-41 | 97.0 | HAdV-41 |
| D0007_01 | F | 99 | HAdV-41 | 98.0 | HAdV-41 |
| D0001_01 | F | 99 | HAdV-41 | 97.0 | HAdV-41 |
| D0011_01 | F | 99 | HAdV-41 | 98.0 | HAdV-41 |
| 928O_04 | D | 94 | NIB; Species D (not HAdV-8) | 95.5* | Species D (not HAdV-8) |
| 926O_04 | D | 94 | NIB; Species D (not HAdV-8) | 95.6* | Species D (not HAdV-8) |
| 925O_04 | D | 94 | NIB; Species D (not HAdV-8) | 95.6* | Species D (not HAdV-8) |
| SO4390_04 | D | 94 | NIB; Species D (not HAdV-8) | 95.0* | Species D (not HAdV-8) |
| HAdV isolates | |||||
| C1519_02 | C | 95 | HAdV-1 | 99.0 | HAdV-1 |
| C1662_03 | C | 95 | HAdV-1 | 99.0 | HAdV-1 |
| C1491_02 | C | 95 | HAdV-1 | 99.5 | HAdV-1 |
| C1629_02 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| 4030_96 | B | 74 | HAdV-3 | 99.0 | HAdV-3 |
| 859_96 | E | 99 | HAdV-4 | 92.2 | HAdV-4 |
| 860_96 | E | 99 | HAdV-4 | 89.4 | HAdV-4 |
| 519_93 | E | 99 | HAdV-4 | 90.7 | HAdV-4 |
| 856_96 | E | 99 | HAdV-4 | 92.7 | HAdV-4 |
| C1201_00 | C | 59 | HAdV-6 | 100.0 | HAdV-6 |
| G3093_03 | C | 59 | HAdV-6 | 99.0 | HAdV-6 |
| 615_96 | D | 99 | HAdV-8 | 100.0 | HAdV-8 |
| 636_96 | D | 99 | HAdV-8 | 100.0 | HAdV-8 |
| 647_96 | D | 99 | HAdV-8 | 100.0 | HAdV-8 |
| 841_94 | D | 99 | HAdV-8 | 100.0 | HAdV-8 |
| 43024_02 | D | 99 | HAdV-8 | 98.0 | HAdV-8 |
| C4292_02 | D | 99 | HAdV-8 | 98.5 | HAdV-8 |
| R1650_04 | A | 99 | HAdV-12 | 97.5 | HAdV-12 |
| R1641_04 | A | 99 | HAdV-12 | 97.5 | HAdV-12 |
| C5335_01 | B | 99 | HAdV-21 | 96.5 | HAdV-21 |
| R1647_04 | B | 71 | HAdV-16 | 99.5 | HAdV-16 |
| C1640_03 | A | 99 | HAdV-31 | 98.5 | HAdV-31 |
| G1T4_03 | D | 94 | NIB; species D (not HAdV-8) | 97.0* | Species D (not HAdV-8) |
| 594_89 | D | 94 | NIB; species D (not HAdV-8) | 96.1* | Species D (not HAdV-8) |
| C1167_00 | D | 94 | NIB; species D (not HAdV-8) | 96.6* | Species D (not HAdV-8) |
a
Species identified by bootstrap.
b
NIB, serotype not identified by bootstrap.
c
*, average score from all members of the group. Boldfacing indicates that the Needleman Wunsch (NW) score for the second-highest serotype was higher than 93%. Due to their close relationship, all cases of HAdV-2 and -6 present this situation. In all cases the highest score pointed to the correct serotype.