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. 2005 Dec;43(12):6007–6014. doi: 10.1128/JCM.43.12.6007-6014.2005

TABLE 3.

Correlation of N. meningitidis MLST sequence types directly from clinical specimens and isolates from the same patients

Clinical-sample no. CT valuea Sample typeb Phenotypec Allele nucleotide sequence no.d
Sequence type from clinical sample Sequence type from cultured isolate ET-37 complex/ET15 clone
abcZ adk aroE fumC gdh pdhC pgm
1 18.37 CSF B:4:P1.4 3 6 9 5 9 6 9 41 41
2 20.05 EDTA B:NT:P1.4 3 6 9 5 9 6 9 41 41
3 20.64 CSF B:15:P1.7,16 4 10 12 4 6 3 8 1100 1100
4 22.11 EDTA C:2a:NT 2 3 4 3 8 4 6 11 11 ET-15
5 22.75 EDTA B:1:P1.4 3 6 9 5 9 6 9 41 41
6 24.87 EDTA C:2b:P1.5,2 2 3 7 2 8 5 2 8 8
7 24.99 EDTA B:1:P1.4 3 6 9 5 9 6 9 41 41
8 26.17 EDTA B:15:P1.7,2 4 10 5 40 6 3 8 259 259
9 26.53 EDTA B:4:P1.4 3 6 9 5 11 6 9 154 154
10 28.04 EDTA W135:2a:P1.5,2 2 3 4 9 8 4 6 11 11 ET-37
11 28.13 EDTA B:22:P1.14 7 8 10 19 10 143 2 1102 1102
12 30.19 EDTA B:1:NT 3 6 9 5 9 6 9 41 41
13 30.39 EDTA B:2a:P1.5 2 3 4 3 8 4 6 11 11 ET-15
14 32.02 EDTA C:2a:P1.5,2 2 3 4 3 8 4 6 11 11 ET-15
15 32.30 EDTA C:2a:P1.5 2 3 4 3 8 4 6 11 11 ET-15
16 32.36 CSF C:2a:NT 2 3 4 3 8 4 6 11 11 ET-15
17 32.42 EDTA B:NT:P1.7 4 10 15 9 8 11 9 269 269
18 32.72 EDTA C:2a:P1.5,2 2 3 4 3 8 4 6 11 11 ET-15
19 34.00 EDTA C:2a:P1.5 2 3 4 3 8 4 6 11 11 ET-15
20 34.19 EDTA C:2a:P1.5 7 5 1 13 36 53 15 213 213
a

The CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence; a value of >45.0 is deemed to be negative.

b

CSF, cerebrospinal fluid; EDTA, blood sample collected in a BD Vacutainer (Becton Dickinson, Plymouth, United Kingdom) containing ethylenediaminetetraacetic acid.

c

Serogroup:serotype:serosubtype.

d

Allele nucleotide sequence number in the MLST database (http://pubmlst.org/neisseria).