TABLE 5.
Case cluster B: MLST results for a cluster of MenB disease in a school during spring 2001
| Sample no. | CT valuea from ctrA screening test | Sample typeb | Phenotypec | siaD groupd | Seqsubtypee | Allele nucleotide sequence no.g
|
MLST classification | School yrf | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| abcZ | adk | aroE | fumC | gdh | pdhC | pgm | ||||||||
| B1 | 32.69 | EDTA | B | P1.19-1,15-11 | 4 | 10 | 15 | 9 | 8 | 11 | 9 | ST-269 (ST-269 complex) | 10 | |
| B2 | 35.14 | EDTA | B | P1.19-1,15-11 | 4 | 10 | 15 | 9 | 8 | 11 | 9 | ST-269 (ST-269 complex) | 10 | |
| B3 | Culture | B:NT:P1.15 | P1.19-1,15-11 | 4 | 10 | 15 | 9 | 8 | 11 | 9 | ST-269 (ST-269 complex) | 11 | ||
| B4 | Culture | B:NT:P1.15 | P1.17-1,23 | 9 | 20 | 9 | 9 | 9 | 6 | 2 | ST-1097 (ST-44 complex) | 11 | ||
| B5 | Culture | B:NT:P1.15 | P1.19,15-1 | 12 | 6 | 9 | 9 | 9 | 9 | 9 | ST-1169 (ST-44 complex) | 7 | ||
| B6 | Culture | B:NT:P1.15 | P1.19,15-1 | 12 | 6 | 9 | 9 | 9 | 6 | 9 | ST-43 (ST-44 complex) | 7 | ||
| B7 | Culture | NG:NT:P1.15 | P1.19-1,15-11 | 4 | 10 | 15 | 9 | 8 | 11 | 9 | ST-269 (ST-269 complex) | 9 | ||
| B8 | Culture | NG:NT:P1.15 | P1.19-3,15 | 8 | 26 | 9 | 24 | 26 | 20 | 18 | ST-212 | 8 | ||
| B9 | Culture | NG:NT:P1.15 | P1.19,15 | 7 | 16 | 55 | 10 | 26 | 56 | 142 | ST-1186 (ST-962 complex) | Staff | ||
| B10 | Culture | B:4Z:P1.15 | P1.17,16-3 | 122 | 6 | 9 | 17 | 9 | 6 | 16 | ST-1166 (ST-44 complex) | 10 | ||
| B11 | Culture | B:4Z:P1.15 | P1.22-1,14 | 4 | 10 | 16 | 9 | 6 | 21 | 3 | ST-1167 | 8 | ||
| B12 | Culture | B:4Z:P1.15 | P1.19,15 | 10 | 10 | 9 | 9 | 9 | 6 | 2 | ST-1168 (ST-44 complex) | 11 | ||
| B13 | Culture | NG:21:P1.15 | P1.18-1,30 | 16 | 2 | 159 | 92 | 77 | 25 | 112 | ST-1117 | 7 | ||
| B14 | Culture | B:1:P1.15 | P1.22,14 | 7 | 5 | 1 | 13 | 36 | 53 | 15 | ST-213 | 11 | ||
The CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence.
EDTA, blood sample collected in a BD Vacutainer (Becton Dickinson, Plymouth, United Kingdom) containing ethylenediaminetetraacetic acid; Culture, organism isolated from throat swab.
Serogroup:serotype:serosubtype (determined by monoclonal antibodies).
Group determined using the siaD TaqMan assay.
Subtype determined by the sequencing of porA variable regions 1 and 2.
Year 7, age 11 to 12; year 8, age 12 to 13; year 9, age 13 to 14; year 10, age 14 to 15; year 11, age 15 to 16.
Allele nucleotide sequence number in MLST database (http://pubmlst.org/neisseria).