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. 2005 Dec;43(12):6048–6053. doi: 10.1128/JCM.43.12.6048-6053.2005

FIG. 1.

FIG. 1.

Schematic representation of the plcABC and plcD loci and the locations of the PCR primers (small arrows), PvuII restriction sites (triangles), and Southern hybridization probes (small black bars). PvuII restriction sites were obtained from the sequence of M. tuberculosis CDC1551 in GenBank. (A) plcCBA gene map of CDC1551 (GenBank accession no. NC_002755). (B) plcD gene map of CDC1551 (accession no. NC_002755). Genes between IS6110 and MT1817 are omitted due to space limit. Primers and sequences are as follows: CPF, 5′-AAGCCGAAATACACGAGGGAGAGC-3′; CPR, 5′-GGGCGGCAAAGGCGGACCAAGAG-3′; BPF, 5′-CGGCAGGCAGGCGGAATCAGAACA-3′; BPR, 5′-TCCGGCGAATGCACCTTGGCTCAC-3′; APF, 5′-GCAGGAAGGCAGGGCAAGTG-3′; APR, 5′-TCGAACGCCGGGAGATTACC-3′; DP1F, 5′-CGCGCGCCGCCGCGCCGAAATA-3′; DP1R, 5′-TCGCCCGGACAGGTCAACAAGGTG-3′; DP2F, 5′-CGCTGGTACACCTGGGGAATCTGGTGACGTAGA-3′; DP2R, 5′-GTCCACTGTCGCCCGGACAGGTCAACAAGGTGT-3′; DP3F, 5′-ACACGAGACATTTGGGCTAG-3′; DP3R, 5′-CAATGCGGATATCAGTGGAC-3′.