TABLE 1.
Numbers and most common locations of IS6110 insertion sites found in four plc genes of M. tuberculosis clinical strains collected from Arkansas between 1 January 1996 and 31 December 2000
| Gene (no.) | No. of IS6110 insertion loci | Sequence of the most common insertion sitea | Position of the most common insertion siteb | Frequency (%)c |
|---|---|---|---|---|
| plcD (n = 144) | 27 | GGTCACACTG↓CGCCGCCAAG | 1978100 | 29/122 (23.77) |
| plcA (n = 13) | 6 | CAGCTTCGGC↓AGCGCTCCCA | 2626532 | 4/11 (36.36) |
| plcB (n = 9) | 5 | ACCGTCGCGT↓CCCGCCAAGC | 2624861 | 5/9 (55.56) |
| plcC (n = 27) | 16 | GGACCGGGTG↓GTCCAGATTG | 2623172 | 3/21 (14.28) |
a
A 3- to 4-bp duplication at the site of the IS6110 insertion is shown with underlining. This duplication happened when IS6110 was inserted without a partial deletion of the plc genes and adjacent regions.
b
The genome sequence of CDC1551 was used as a reference.
c
The number of nominators and denominators was counted by strain, which was defined by RFLP and pTBN12 genotyping.