Representative multiplex PCR test using primers 406, 408, and 649 (Table 1) with chromosomal DNA isolated from various M. catarrhalis isolates. Lanes 1 to 3 show amplicons from the three LOS serotype control strains: LOS serotype A, ATCC 25238 (lane 1); LOS serotype B, 7169 (lane 2); and LOS serotype C, RS10 (lane 3). Lanes 4 to 8 contain amplicons from five COPD patients' isolates: 3P3B1 (lane 4), 51P9B1 (lane 5), 5P26B1 (lane 6), 18P6B1 (lane 7), and 12P80B1 (lane 8). Lanes 9 to 13 contain amplicons from five children's OME isolates: HF-039 (lane 9), HF-2246 (lane 10), HF-165 (lane 11), HF-218 (lane 12), and HF-006 (lane 13). Lanes 14 to 18 contain amplicons from five geographically diverse (global) isolates: 2951 (lane 14), 035E (lane 15), CCUG 3292 (lane 16), ATCC 43617 (lane 17), and CCUG 26391 (lane 18).