Fig. 6. Functional analysis of the Dlx3 promoter CCAAT box motif.

A, the diagram illustrates the p84CAT and p34CAT deletion constructs and the sequence of the mutation substituting the CCAAT box motif. B, primary mouse keratinocytes were transfected with p84CAT, p84mCAT, and p34CAT and incubated with medium containing 0.05 or 1.4 mm Ca²⁺. After 48 h, cells were harvested. The CAT activity was normalized to the protein concentration measured by the Bradford assay. The bars represent the average normalized CAT activity of three experiments done on duplicate plates for each construct.