Skip to main content
. 2005 Dec;71(12):8183–8190. doi: 10.1128/AEM.71.12.8183-8190.2005

FIG. 3.

FIG. 3.

(A) Products of nifH amplified by means of RT-PCR using total RNA extracted from free-living Herbaspirillum sp. B501gfp1 cultured under different oxygen concentrations. Lanes 1 to 8, RNA was extracted from cells cultured under 0.4, 0.6, 1.0, 2.0, 5.0, 8.0, 12.0, and 21.0% (vol/vol) oxygen concentrations, respectively; lane 9, negative control reaction without PCR template. gfp, a pair of gfp primers was used to amplify the gfp transcripts; nif, a pair of nifH primers was used to amplify the nifH transcripts. Total RNA was adjusted to the same amount for the respective RT-PCRs. (B) Quantification of the nifH transcripts of Herbaspirillum sp. B501gfp1, cultured under different oxygen concentrations, by means of real time RT-PCR. (C) Acetylene reduction activity of Herbaspirillum sp. B501gfp1 cultured under different oxygen concentrations.