TABLE 2.
Primers used in the present study
| Application | Primer and sequencea | Reference or source |
|---|---|---|
| Cloning | ||
| 19F | 5′-GCIWTYTAYGGIAARGGIGG-3′ | 34 |
| 407R | 5′-AAICCRCCRCAIACIACRTC-3′ | 34 |
| Sequencing | ||
| −21 M13 | 5′-TGTAAAACGACGGCCAGT-3′ | Applied Biosystems |
| M13 Rev | 5′-CAGGAAACAGCTATGACC-3′ | Applied Biosystems |
| nif370 | 5′-TGTAACACCAGAAAGAAGTG-3′ | Present study |
| nif370-1 | 5′-GGACACCATCCTGTCGCTGG-3′ | Present study |
| nif370-2 | 5′-ACAAGGAACTGGAACTGGCC-3′ | Present study |
| nif370-3 | 5′-TAGCGCAGGTTTGTTTACC-3′ | Present study |
| nif370-4 | 5′-ACATCAGCCATGGTCCGGT-3′ | Present study |
| nif270 | 5′-CTCGACCCAGGGAAATGCC-3′ | Present study |
| nif270-1 | 5′-CTCGTCGACGATCTTTTCCA-3′ | Present study |
| nif270-2 | 5′-TTTCTTCAACTGTGAGGCTC-3′ | Present study |
| nif270-3 | 5′-AGTTGGCGTACTTCAAAATG-3′ | Present study |
| nif270-5 | 5′-TTATTGGTCTTCGTCTCA-3′ | Present study |
| RT-PCR | ||
| nif270-3 | 5′-AGTTGGCGTACTTCAAAATG-3′ | Present study |
| nif370-1 | 5′-GGACACCATCCTGTCGCTGG-3′ | Present study |
| gfp553 | 5′-TCAACTAGCAGACCATTATCAACA-3′ | Present study |
| gfp681 | 5′-ACCATGTGGTCTCTCTTTTCG-3′ | Present study |
| Real-time RT-PCR | ||
| nifH238 | 5′-ATCGGCTACCAGAACATC-3′ | Present study |
| nifH366 | 5′-GTAATCGGTGTCATCATAGG-3′ | Present study |
a
I represents inosine, R represents A or G, W represents A or T, and Y represents C or T.