TABLE 6.

Amino acid changes between the proteins coded by the AGT1 genes in the Saccharomyces Genome Database and the ale and lager strains

TMDb	Amino acida
	Position	SGD	A60	A179	A15	A24
	40	D	N	N	N	N
	45	K	R	R
	59	T		I
	74	V			M	M
	78	M	T	T
	80	A	T	T
	83	D			E	E
	102	K	I	I
	128	S	N	N	N	N
I (164-184)	164	L	Q	Q	Q	Q
	168	M	T	T
	175	T	P	P
	198	I	V	V
II (202-222)	215	I	V	V	V	V
	219	I	M	M	M	M
	226	S	G	G	G	G
	228	A	T	T	T	T
III (241-266)	276	G			D	D
IV (284-311)	333	V	I	I
	359	N	D	D	D	D
	375	T			A	A
	381	S	T	T	T	T
	385	V	C	C	C	C
	392	Y		C
	395	E			Stopc	Stopc
V (405-427)	409	L	V	V	V	V
VI (432-459)	448	I	V/Id	I/Vd	V	V
	459	S	G	G		G
VII (461-489)	488	A	T	T
VIII (504-532)	509	L		I
	548	V	A
	556	T		S
	595	A		D

^aAmino acids are shown at positions where the SGD and brewer's yeast protein sequences differ; no entry means the amino acid is the same as in the SGD.

^bThe positions of transmembrane domains (TMDs) indicated in SGD are shown.

^cThe sequences of the lager strains' hypothetical proteins after the Stop codons were obtained by correcting the frameshift.

^dResidue 448 was the same as in the SGD in 3 of 13 A60 sequences and 10 of 17 A179 sequences.