TABLE 2.
Gene positiona | Used in seven-locus MLST | Genea | Gene functiona | Biochemical functiona | Primer sequences (forward/reverse) | Gene length (bp) |
---|---|---|---|---|---|---|
XF0136 | Yes | holC | DNA polymerase III holoenzyme, chi subunit | Replication | 5′-GATTTCCAAACCGCGCTTTC-3′/5′-TCATGTGCAGGCCGCGTCTCT-3′ | 342 |
XF0257 | rfbD | dTDP-4-dehydrorhamnose-3, 5- epimerase | Surface polysaccharides | 5′-TTTGGTGATTGAGCCGAGGGT-3′/5′-CCATAAACGGCCGCTTTC-3′ | 429 | |
XF0316 | Yes | nuoL | NADH-ubiquinone oxidoreductase, NQO12 subunit | Aerobic respiration | 5′-CATTATTGCCGGATTGTTAGG-3′/5′-GCGGGAAACATTACCAAGC-3′ | 1,821 |
XF0318 | nuoN | NADH-ubiquinone oxidoreductase, NQO14 subunit | Aerobic respiration | 5′-GGGTTAAACATTGCCGATCT-3′/5′-CGGGTTCCAAAGGATTCCTAA-3′ | 1,311 | |
XF0656 | Yes | gltT | Glutamate symport protein | Transport of amino acids | 5′-TTGGGTGTGGGTACGTTGCTG-3′/5′-CGCTGCCTCGTAAACCGTTGT-3′ | 951 |
XF0832 | Yes | cysG | Siroheme synthase | Biosynthesis of heme, porphyrin | 5′-GGCGGCGGTAAGGTTG-3′/5′-GCGTATGTCTGTGCGGTGTGC-3′ | 1,170 |
XF0910 | Yes | petC | Ubiquinol cytochrome c oxidoreductase, cytochrome c1 subunit | Electron transport | 5′-CTGCCATTCGTTGAAGTACCT-3′/5′-CGTCCTCCCAATAAGCCT-3′ | 531 |
XF1632 | pilU | Twitching motility protein | Surface structures | 5′-CAATGAAGATTCACGGCAATA-3′/5′-ATAGTTAATGGCTCCGCTATG-3′ | 873 | |
XF1818 | Yes | leuA | 2-Isopropylmalate synthase | Amino acid biosynthesis | 5′-GGGCGTAGACATTATCGAGAC-3′/5′-GTATCGTTGTGGCGTACACTG-3′ | 1,218 |
XF2447 | Yes | lacF | ABC transporter sugar permease | Transport of carbohydrates | 5′-TTGCTGGTCCTGCGGTGTTG-3′/5′-CCTCGGGTCATCACATAAGGC-3′ | 642 |
Information based on information reported in reference 39.