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. 2005 Dec;71(12):8491–8499. doi: 10.1128/AEM.71.12.8491-8499.2005

TABLE 2.

Positions, functions, PCR primers, and lengths sequenced for the 10 X. fastidiosa genes

Gene positiona Used in seven-locus MLST Genea Gene functiona Biochemical functiona Primer sequences (forward/reverse) Gene length (bp)
XF0136 Yes holC DNA polymerase III holoenzyme, chi subunit Replication 5′-GATTTCCAAACCGCGCTTTC-3′/5′-TCATGTGCAGGCCGCGTCTCT-3′ 342
XF0257 rfbD dTDP-4-dehydrorhamnose-3, 5- epimerase Surface polysaccharides 5′-TTTGGTGATTGAGCCGAGGGT-3′/5′-CCATAAACGGCCGCTTTC-3′ 429
XF0316 Yes nuoL NADH-ubiquinone oxidoreductase, NQO12 subunit Aerobic respiration 5′-CATTATTGCCGGATTGTTAGG-3′/5′-GCGGGAAACATTACCAAGC-3′ 1,821
XF0318 nuoN NADH-ubiquinone oxidoreductase, NQO14 subunit Aerobic respiration 5′-GGGTTAAACATTGCCGATCT-3′/5′-CGGGTTCCAAAGGATTCCTAA-3′ 1,311
XF0656 Yes gltT Glutamate symport protein Transport of amino acids 5′-TTGGGTGTGGGTACGTTGCTG-3′/5′-CGCTGCCTCGTAAACCGTTGT-3′ 951
XF0832 Yes cysG Siroheme synthase Biosynthesis of heme, porphyrin 5′-GGCGGCGGTAAGGTTG-3′/5′-GCGTATGTCTGTGCGGTGTGC-3′ 1,170
XF0910 Yes petC Ubiquinol cytochrome c oxidoreductase, cytochrome c1 subunit Electron transport 5′-CTGCCATTCGTTGAAGTACCT-3′/5′-CGTCCTCCCAATAAGCCT-3′ 531
XF1632 pilU Twitching motility protein Surface structures 5′-CAATGAAGATTCACGGCAATA-3′/5′-ATAGTTAATGGCTCCGCTATG-3′ 873
XF1818 Yes leuA 2-Isopropylmalate synthase Amino acid biosynthesis 5′-GGGCGTAGACATTATCGAGAC-3′/5′-GTATCGTTGTGGCGTACACTG-3′ 1,218
XF2447 Yes lacF ABC transporter sugar permease Transport of carbohydrates 5′-TTGCTGGTCCTGCGGTGTTG-3′/5′-CCTCGGGTCATCACATAAGGC-3′ 642
a

Information based on information reported in reference 39.