TABLE 2.
Fatty acid profile of strain 10403S and Bkd mutant cld-2 in BHI with various supplements at 37°C and 10°C
| Strain, supplement, and temp | % (wt/wt) of total fatty acida
|
Anteiso: iso ratio | ACCLd | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Anteiso-C13:0 | Iso-C14:0 | Iso-C15:0 | Anteiso-C15:0 | Iso-C16:0 | Iso-C17:0 | Anteiso-C17:0 | |||
| 10403S, none, 37°C | NDb | 0.8 | 10.1 | 46.8 | 2.4 | 3.2 | 35.7 | 5 | 15.8 |
| 10403S, none, 10°C | 0.3 | 1.1 | 12.9 | 67.7 | 1.5 | 0.8 | 14.9 | 5.1 | 15.3 |
| 10403S, cerulenin, 37°C | 1.3 | ND | 9.4 | 70.1 | 1.3 | ND | 16.7 | 8.2 | 15.3 |
| cld-2, short BCFAsc, 37°C | ND | 8.5 | 5.8 | 38.0 | 22.6 | 1.3 | 17.4 | 1.5 | 15.6 |
| cld-2, short BCFAs, 10°C | ND | 16.3 | 7.1 | 52.7 | 11.9 | ND | 6.2 | 1.7 | 15.1 |
a
Some minor fatty acid components are not shown in this table.
b
ND, not detected.
c
Short BCFAs 2-methylbutyrate, isobutyrate, and isovalerate were added at 0.1 mM each.
d
ACCL, average carbon chain length (calculated as described for Table 1).