TABLE 3.
AFLP markers generated on S. glossinidius strains from G. morsitans morsitans using five primer pair combinations
| No. | Primer pairc | Total no. of markers | No. of polymorphic markers | % Polymorphic markers |
|---|---|---|---|---|
| I | EcoRI-AG*a/MseI-0 | 24 | 2 | 8.3 |
| II | EcoRI-AG*a/MseI-C | 15 | 0 | 0 |
| III | EcoRI-C*b/MseI-0 | 55 | 6 | 10.9 |
| IV | EcoRI-C*b/MseI-C | 35 | 2 | 5.7 |
| V | EcoRI-0/MseI-C*b | 36 | 0 | 0 |
| Total | 165 | 10 | 6 |
a
Primer labeled with infrared dye IRD800.
b
Primer labeled with infrared dye IRD700.
c
Letters after the restriction enzyme name represent the nucleotide base(s) added to each primer to select only a subset of the fragments using PCR amplification. A zero indicates that no base was added. *, primer labeled for detection.