TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− (φ80dlacZ ΔM15) Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) supE44 λ−thi-1 gyrA relA | 16 |
| BL21(DE3) | F−ompT rB− mB−1 DE3 | 45 |
| BW25141 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) lacIp-4000(lacIq) Δ(phoB-phoR) 580 λ−galU95 ΔuidA3::pir+recA1 endA9(del-ins)::FRT rph-1 Δ(rhaD-rhaB)568 rrnB-3 hsdR514 | 14 |
| EC100DTMpir-116 | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ−rpsL nupG pir-116(DHFR) | Epicentre Technologies |
| C. jejuni | ||
| NCTC 11168 | Clinical isolate used for genome sequencing | 34 |
| 81-176 | Clinical isolate | 26 |
| M1 | Environmental isolate | D. G. Newell, gift |
| Plasmids | ||
| pET29b+C9 | pET29b+ carrying Q131R and E137K mutations in Himar1 transposase | 29 |
| pRY111 | Source of campylobacter cat cassette | 53 |
| pAJG39 | pEnterprise2 with 0.6-kb fragment harbouring oriR6Kγ from EZ::TN<R6Kγori/KAN-2> cloned into the SalI site | This study |
| pAJG40-134 | pAJG39 with 0.1-kb fragment harbouring an individual Holden DNA tag cloned into the PstI site | This study |
| pEnterprise2 | pUC19 with 1.5-kb fragment harbouring cat and transposon from pEnterprise, cloned as a PmeI fragment into blunt-ended EcoRI and HindIII sites | D. R. Hendrixson, gift; reference 17 |
| EZ::TN<R6Kγori/KAN-2> | Source of the R6Kγ origin of replication | Epicentre Technologies |