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. 2005 Dec;71(12):8911–8919. doi: 10.1128/AEM.71.12.8911-8919.2005

FIG. 3.

FIG. 3.

Effect of the addition of β-ME on aerobic growth of strain NZ9000(pFL010) and strain NZ9000(pNZ8148). Different concentrations of β-ME (⋄, 0 mM; □, 30 mM; ▵, 60 mM) were added to 250-ml flasks containing 15 ml of M17 broth. Precultures of L. lactis were inoculated to achieve an initial cell number of (1.0 ± 0.5) × 107 CFU/ml. Cultivations were performed on a shaker at 220 rpm for 24 h, during which the time course of biomass and viable cells of both cultures were measured. (A) Strain NZ9000(pFL010) biomass. (B) Strain NZ9000(pFL010) viable cells. (C) Strain NZ9000 (pNZ8148) biomass. (D) Strain NZ9000(pNZ8148) viable cells. Results are expressed as the means of duplicate measurements on three independent samples. The standard deviations were lower than 5% of the values (for biomass measurements) or 15% of the values (for CFU measurements).