TABLE 3.
Growth characteristics of strain NZ9000(pFL010) and NZ9000(pNZ8148)a
| Strain | Biomass in M17 broth (g/liter)
|
Biomass in CM (g/liter)
|
||
|---|---|---|---|---|
| Aerobic | Static | Aerobic | Static | |
| NZ9000(pFL010) | 4.46 ± 0.05 | 0.97 ± 0.03 | 4.02 ± 0.06 | 0.88 ± 0.03 |
| NZ9000(pNZ8148) | 0.97 ± 0.01 | 1.20 ± 0.01 | 0.31 ± 0.01 | 0.33 ± 0.02 |
a
Growth experiments were performed in triplicate in 250-ml flasks containing 15 ml of M17 broth or CM. Both strains were grown in M17 broth for 16 h, and the cultures were used to inoculate fresh M17 broth or CM. Aerobic cultures were cultivated on a reciprocal shaker with a rotation speed of 200 rpm. All cultivations were for 12 h at 30°C.