TABLE 4.
Clone groupa | T-RF (bp) | Accession no. | General phylogenetic position | % of clonesb
|
|||
---|---|---|---|---|---|---|---|
Outlet | 390 m | 550 m | 648 m | ||||
D8A-1 | 209 | AY768821 | Low-G+C, gram-positive Desulfotomaculum | 36 | NA | 100 | 98 |
D8A-2 | 93 | AY768822 | Unresolved within low-G+C, gram-positive group | 0 | NA | 0 | 1 |
D8A-3 | 239 | AY768823 | Unresolved within low-G+C, gram-positive group | 1 | NA | 0 | 0 |
D8A-4 | 69 | AY768824 | Betaproteobacteria, Comamonadaceae | 63 | NA | 0 | 0 |
D8A-5 | 69 | AY768825 | Betaproteobacteria, Comamonadaceae | 0 | NA | 0 | 1 |
D8A-6 | 197 | AY768826 | Euryarchaeota, Methanobacteriaceae | 0 | NA | 3 | 0 |
D8A-7 | 197 | AY768827 | Euryarchaeota, Methanobacteriaceae | 86 | NA | 95 | 97 |
D8A-8 | 335 | AY768828 | Euryarchaeota, Methanobacteriaceae | 14 | NA | 3 | 3 |
D8A-mcrA1 | NA | AY768819 | Euryarchaeota, Methanobacteriaceae | 93.5 | 100 | NA | 100 |
D8A-mcrA2 | NA | AY768820 | Euryarchaeota, Methanobacteriaceae | 6.5 | 0 | NA | 0 |
D8A-dsr1 | NA | AY768818 | Low-G+C, gram-positive Desulfotomaculum | NA | NA | 100 | NA |
Ninety-six clones were screened for each rRNA library, and 48 clones were screened for the mcrA and dsrAB libraries.
NA, not analyzed.