FIG. 4.

DE values generated from type strain data set containing 2,022 16S rRNA gene sequences without any degenerate base positions (see text). DE value was generated for each of the 2,043,231 pairwise sequence comparisons and plotted against evolutionary distance between sequences. (A) The data set prior to the removal of the 15 anomalous sequences (see text); (B) the plot after removal; (C) the quantile values used to describe these data and incorporated into the Pintail program as a means of calibration.