TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Rhodococcus opacus TKN14 | Gram-positive bacterium that grows on o-xylene | MBIC 05572 |
| Escherichia coli XL1-Blue MR | Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| E. coli JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZ ΔM15] | Takara |
| Plasmids | ||
| SuperCos 1 | Stratagene | |
| pBluescript II SK(+) | Stratagene | |
| pT7Blue T-vector | Novagen | |
| pTrcHisA | Invitrogen | |
| pRTKN700 | Apr; 35-kb Sau3AL-partially digested genome DNA from R. opacus TKN14 in SuperCOS 1 | This study |
| pNot1-9 | Apr; 15-kb NotI fragment from pRTKN700 in pBluescript II SK+ | This study |
| pN54-1-5 | Apr; 7.26-kb PCR fragment from the 15-kb NotI fragment in pT7Blue T-vector | This study |
| pNidABCD | Apr; 4.59-kb EcoRI fragment from the 15-kb NotI fragment in pBluescript II SK+ | This study |
| nidA-disruptant | Apr; pN54-1-5 with disrupted nidA | This study |
| nidB-disruptant | Apr; pN54-1-5 with disrupted nidB | This study |
| ORF1-disruptant | Apr; pN54-1-5 with disrupted ORF1 | This study |
| ORF2-3-disruptant | Apr; pN54-1-5 with disruption in both ORF2 and ORF3 | This study |
| pTrcNidAB | Apr; nidAB (1.94 kb) in pTrcHisA | This study |
| pTrcRd | Apr; Rd (0.28 kb) in pTrcHisA | This study |
| pTrcNidABRd | Apr; nidAB and Rd (2.22 kb) in pTrcHisA | This study |
| pTrcNidABRdORF4 | Apr; nidAB, Rd, and ORF4 (2.82 kb) in pTrcHisA | This study |
| pTrcNidABRdORF5 | Apr; nidAB, Rd, and ORF5 (2.91 kb) in pTrcHisA | This study |
| pTrcNidABRdORF6 | Apr; nidAB, Rd, and ORF6 (2.85 kb) in pTrcHisA | This study |
| pTrcNidABRdORF789 | Apr; nidAB, Rd, ORF7, ORF8; and ORF9 (3.68 kb) in pTrcHisA | This study |
| pTrcNidABRdORF8 | Apr; nidAB, Rd, and ORF8 (2.73 kb) in pTrcHisA | This study |
| pTrcNidABRdORF9 | Apr; nidAB, Rd, and ORF9 (2.94 kb) in pTrcHisA | This study |
| pTrcNidABRdORF78 | Apr; nidAB, Rd, ORF7, and ORF8 (2.93 kb) in pTrcHisA | This study |
| pTrcNidABRdORF89 | Apr; nidAB, Rd, ORF8, and ORF9 (3.45 kb) in pTrcHisA | This study |
| pTrcNidABORF8 | Apr; nidAB and ORF8 (2.45 kb) in pTrcHisA | This study |