TABLE 2.
Relative activities of the remaining selected mutants
| Amino acid substitution(s) | Relative activitya (%) with:
|
|
|---|---|---|
| 1 mM PenG | 10 mM PenG | |
| Wild type | 100 | 160 |
| G300V | 410 | 370 |
| M73T/I277Q | 350 | 350 |
| M73T/C281Y | 610 | 530 |
| V275I/C281Y | 260 | 310 |
| V275I/I305L | 310 | 330 |
| L277Q/I305M | 610 | 520 |
| M73T/G300V/I305L | 640 | 380 |
| G79E/V275I/C281Yb | 430 | 200 |
| G79E/V275I/I305Lb | 470 | 240 |
| G79E/V275I/I305Mb | 1,110 | 540 |
| M188V/V2751/G300V | 440 | 310 |
| V275I/C281Y/G300V | 620 | 380 |
| V275I/C281Y/I305Mb | 1,290 | 690 |
| V275I/N304K/I305Lb | 300 | 130 |
| C281Y/N304K/I305Mb | 650 | 230 |
| A11V/T91A/C281Y/I305L | 220 | 200 |
| G79E/V275I/C281Y/I305Lb | 250 | 90 |
| Y184H/M188I/C281Y/I305L | 610 | 460 |
| Y184H/H244Q/T259I/L277Q | 320 | 310 |
| M73T/I118V/A140V/H244Q/C281Y | 450 | 350 |
| A106T/E169K/V275I/C281Y/I305L | 450 | 380 |
| R135Q/C155Y/R179Q/M188V/I305M | 360 | 290 |
| E144K/M188I/A198T/V275I/C281Y | 160 | 200 |
a
The relative activity was determined using 1 and 10 mM PenG, and the activity of the wild-type enzyme with 1 mM PenG was defined as 100%.
b
DAOCS mutant obtained in previous studies (31).