TABLE 2.
Representative rhizosphere and endophytic strains found in association with poplar plants that were inoculated with B. cepacia BU61 or VM1468
| Straina | Origin | Species | Toluene phenotype | tomA4 PCR | BOX-PCR pattern |
|---|---|---|---|---|---|
| BU61 | Control | B. cepacia | Tol+ | + | Unique |
| VM1468 | Control | B. cepacia | Tol+ | + | Unique |
| W604 | Root, VM1468 inoculum | Enterobacter sp. | Tol+ | + | Similar to S662, S671, R558-1 |
| W607 | Root, VM1468 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to S649, W635, R551-3 |
| W619 | Root, VM1468 inoculum | Pseudomonas putida | Tol+ | + | Similar to W630, W645, S672, R599 |
| W630 | Root, BU61 inoculum | Pseudomonas putida | Tol+ | + | Similar to W619, W645, S672, R599 |
| W633 | Root, BU61 inoculum | Ochrobactrum sp. | Tol+ | + | Unique |
| W635 | Root, BU61 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to W607, S649, R551-3 |
| S645 | Stem, VM1468 inoculum | Pseudomonas putida | Tol+ | + | Similar to W619, W630, S672, R599 |
| S649 | Stem, VM1468 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to W607, R551-3 |
| S656 | Stem, VM1468 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to R572, R591 |
| S662 | Stem, VM1468 inoculum | Enterobacter sp. | Tol+ | + | Similar to W604, S671, R558-1 |
| S671 | Stem, BU61 inoculum | Enterobacter sp. | Tol+ | + | Similar to W604, S662, R558-1 |
| S672 | Stem, BU61 inoculum | Pseudomonas putida | Tol+ | + | Similar to W619, W630, S645, R599 |
| T675 | Twig, VM1468 inoculum | B. cepacia | Tol+ | + | Unique |
| R571 | Rhizosphere, VM1468 inoculum | Pseudomonas putida | Tol+ | + | Unique |
| R572 | Rhizosphere, VM1468 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to S656, R591 |
| R591 | Rhizosphere, BU61 inoculum | Stenotrophomonas maltophilia | Tol+ | + | Similar to R572, S656 |
| R599 | Rhizosphere, BU61 inoculum | Pseudomonas putida | Tol+ | + | Similar to W619, W630, S645, S672 |
| R551-2 | Rhizosphere, no inoculum | Pseudomonas putida | Tol+ | − | Unique |
| R551-3 | Rhizosphere, no inoculum | Stenotrophomonas maltophilia | Tol− | − | Similar to W607, W635, S649 |
| R557-2 | Rhizosphere, no inoculum | Pseudomonas sp. | Tol− | − | Unique |
| R558-1 | Rhizosphere, no inoculum | Enterobacter sp. | Tol− | − | Similar to W604, S662, S671 |
| R558-2 | Rhizosphere, no inoculum | Pseudomonas putida | Tol− | − | Unique |
a
Strains were selected from 284 medium plates with toluene as the sole carbon source. Distinct strains from various samples were compared based on their different BOX-PCR patterns. Strains were identified based on the sequences of their 16S rRNA genes. PCR was used to confirm the presence of the tomA4 gene. Cultivable bacteria from noninoculated control plants that were isolated on 284 gluconate medium plates were characterized as controls.