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. 2005 Dec;71(12):8970–8973. doi: 10.1128/AEM.71.12.8970-8973.2005

TABLE 3.

Trypsin sensitivity of HMW bacteriocinsa

Strain Activity (AU) after 30 min of incubation at trypsin concn (% wt/vol) of:
0b 0.05 0.1
Pragia fontium strains
    24342 25 24 20
    24613 28 28 24
    24647 26 24 22
Budvicia aquatica 24522 25 23 21
a

One-milliliter samples of phage tail-like bacteriocin suspensions in TY broth were mixed with stock trypsin solutions (in H2O) in prewarmed Eppendorf tubes and incubated at 37°C. Samples were serially diluted (2−1 to 2−8) with TY broth, and drops of all dilutions were placed on agar plates (1.5% [wt/vol] nutrient agar no. 2; Imuna, Slovakia) overlaid with a corresponding indicator strain in soft agar 0.7% (wt/vol). AU, arbitrary units.

b

Control.