TABLE 3.
Strain | Activity (AU) after 30 min of incubation at trypsin concn (% wt/vol) of:
|
||
---|---|---|---|
0b | 0.05 | 0.1 | |
Pragia fontium strains | |||
24342 | 25 | 24 | 20 |
24613 | 28 | 28 | 24 |
24647 | 26 | 24 | 22 |
Budvicia aquatica 24522 | 25 | 23 | 21 |
One-milliliter samples of phage tail-like bacteriocin suspensions in TY broth were mixed with stock trypsin solutions (in H2O) in prewarmed Eppendorf tubes and incubated at 37°C. Samples were serially diluted (2−1 to 2−8) with TY broth, and drops of all dilutions were placed on agar plates (1.5% [wt/vol] nutrient agar no. 2; Imuna, Slovakia) overlaid with a corresponding indicator strain in soft agar 0.7% (wt/vol). AU, arbitrary units.
Control.