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Examples of urease inhibition in agar diffusion assay. (A) Control. One hundred microliters of sterile water was added to the paper disk. (B) One hundred microliters of phytochemical extracts was added into the paper disk. All extract mixtures were tested at the level of 0.05 mg phenolics per disk for urease inhibition. The plate medium was adjusted to pH 6.0. The yellow zone indicates the area where there is no urease activity. The purple zone indicates the area where urease is active.
