TABLE 1.

Substrate specificity of native GOOX

Substrate	Relative activitya		Kmb (mM)	kcat (min−1)	kcat/Km (mM−1 min−1)
	0.2 mM maltose	10 mM maltose
Glucose	0.2	3.5	8.12 ± 0.16	546 ± 16	67
Lactose	6.4	3.8	0.066 ± 0.008	819 ± 25	12,400
Maltose	1.0	6.0	2.47 ± 0.05	531 ± 16	215
Maltotriose	1.1	6.2	1.11 ± 0.02	385 ± 12	348
Maltotetraose	0.4	4.3	2.51 ± 0.05	388 ± 12	155
Maltopentaose	0.2	2.6	10.6 ± 0.12	618 ± 18	58
Maltohexaose	0.2	3.1	2.60 ± 0.05	276 ± 11	106
Maltoheptaose	0.2	2.3	6.95 ± 0.15	415 ± 13	60
Cellobiose	7.7	2.8	0.048 ± 0.08	322 ± 11	6,710
Cellotriose	7.4	2.1	0.026 ± 0.002	776 ± 16	29,800
Cellotetraose	7.5	2.0	0.012 ± 0.002	200 ± 10	16,700
Cellopentaose	7.6	1.3	0.026 ± 0.003	239 ± 11	9,190
Cellohexaose	7.2	1.1	0.069 ± 0.007	222 ± 10	3,220

^aThe enzyme activity with 0.2 mM maltose was defined as 1.

^bThe kinetic parameter data are means ± standard errors for three independent experiments.