TABLE 3.
Primer | Sequencea | Purpose |
---|---|---|
GOOX-5′ | GARTTYCAYGARGARGA | Degenerate PCR |
GOOX-3′ | CCRAARAARTTNCCYTCCCARTT | Degenerate PCR |
GOOX-RT1 | GTCCAGGCTTCATGGATCCAAGAAGCGCAACTC | Reverse transcription-PCR |
GOOX-RT2 | AAGCTTCAAGTCTAAATCAG | Reverse transcription-PCR |
GOOX-EX1 | GCTTCATGGATCCAGGAATTCAACTCAATCAACGCCTG | Expression in P. pastoris |
GOOX-EX2 | TTCAAGTCTAAATCATCTAGATAGGCAATGGGCTCAAC | Expression in P. pastoris |
H70A-FOR | AAGGGTGGTGGTGCCAGTTACGGTTCTTATGG | H70A mutant |
H70A-REV | ATAAGAACCGTAACTGGCACCACCACCCTTGG | H70A mutant |
H70S-FOR | AAGGGTGGTGGTTCCAGTTACGGTTCTTATGG | H70S mutant |
H70S-REV | ATAAGAACCGTAACTGGAACCACCACCCTTGG | H70S mutant |
H70C-FOR | AAGGGTGGTGGTTGCAGTTACGGTTCTTATGG | H70C mutant |
H70C-REV | ATAAGAACCGTAACTGCAACCACCACCCTTGG | H70C mutant |
H70Y-FOR | AAGGGTGGTGGTTACAGTTACGGTTCTTATGG | H70Y mutant |
H70Y-REV | ATAAGAACCGTAACTGTAACCACCACCCTTGG | H70Y mutant |
R = A/G; Y = T/C.