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. 2005 Dec;71(12):8881–8887. doi: 10.1128/AEM.71.12.8881-8887.2005

TABLE 3.

Oligonucleotide primers used in this study

Primer Sequencea Purpose
GOOX-5′ GARTTYCAYGARGARGA Degenerate PCR
GOOX-3′ CCRAARAARTTNCCYTCCCARTT Degenerate PCR
GOOX-RT1 GTCCAGGCTTCATGGATCCAAGAAGCGCAACTC Reverse transcription-PCR
GOOX-RT2 AAGCTTCAAGTCTAAATCAG Reverse transcription-PCR
GOOX-EX1 GCTTCATGGATCCAGGAATTCAACTCAATCAACGCCTG Expression in P. pastoris
GOOX-EX2 TTCAAGTCTAAATCATCTAGATAGGCAATGGGCTCAAC Expression in P. pastoris
H70A-FOR AAGGGTGGTGGTGCCAGTTACGGTTCTTATGG H70A mutant
H70A-REV ATAAGAACCGTAACTGGCACCACCACCCTTGG H70A mutant
H70S-FOR AAGGGTGGTGGTTCCAGTTACGGTTCTTATGG H70S mutant
H70S-REV ATAAGAACCGTAACTGGAACCACCACCCTTGG H70S mutant
H70C-FOR AAGGGTGGTGGTTGCAGTTACGGTTCTTATGG H70C mutant
H70C-REV ATAAGAACCGTAACTGCAACCACCACCCTTGG H70C mutant
H70Y-FOR AAGGGTGGTGGTTACAGTTACGGTTCTTATGG H70Y mutant
H70Y-REV ATAAGAACCGTAACTGTAACCACCACCCTTGG H70Y mutant
a

R = A/G; Y = T/C.