TABLE 3.
Effects of inhibitors and metal ions (1 mM) on the activity of recombinant PepO of Bifidobacterium animalis subsp. lactis
| Substance | Mean residual activitya (±SD) |
|---|---|
| Phosphoramidon | 23.46 (±5.92) |
| Diethyl pyrocarbonate | 50.77 (±5.29) |
| Thiorphan | 80.77 (±8.16) |
| EDTA | 5.77 (±6.53) |
| 1,10-Phenanthroline | 0.00 (±0.86) |
| p-(Hydroxymercuri)benzoic acid | 43.85 (±5.77) |
| PMSF | 30.58 (±2.45) |
| Dithiothreitol | 110.12 (±15.37) |
| Zn2+ | 15.77 (±1.33) |
| Mn2+ | 115.77 (±5.45) |
| Cu2+ | 5.77 (±3.26) |
a
Expressed as a percentage of the activity on bradykinin in the absence of metal ions and chemical reagents, taken as 100% (0.064 U/mg).