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Purification of the prolipase from inclusion bodies with a cobalt-based resin. Lane 1, pellet solution; lanes 2 and 3, washings; lanes 4, 5, and 6, elution; lane M, molecular mass marker (66, 45, 36, 29, 24, and 20.1 kDa). Ten-microliter aliquots of the soluble and insoluble fraction samples, prepared as described in the text, were loaded onto each lane. The arrow indicates the prolipase.
