Multiplex PCR analysis of SD isolates. Representative results shown are for multiplex PCR using primers designed to amplify genes encoding alpha toxin, CPB2 and CPE. Migrations of PCR products derived from each toxin gene are indicated on the right. Results are shown for control strains F4969 (a cpe+, type A, non-food-borne GI disease isolate), JGS1807 (a cpe−/cpb2+, type A, pig GI disease isolate), and ATCC 3624 (a cpe−, type A isolate) and representative SD isolates 6263/95, 4178/94, and 4370/94. Molecular sizes of DNA markers are given on the left.