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. 2005 Dec;71(12):8362–8370. doi: 10.1128/AEM.71.12.8362-8370.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 2. — RFLP Southern blot analysis of SD isolates. (A) cpe RFLP Southern blot analysis of NruI-digested DNAs from SD isolates with a 639-bp DIG-labeled cpe-specific probe. Results are shown for control strains NCTC8239 (a chromosomal cpe, food poisoning isolate), F4969 (a plasmid cpe, non-food-borne GI disease isolate), and ATCC 3624 (a cpe-negative isolate) and for representative SD isolates 6253/94, 6257/94, 6263/95, and 7458/94. Molecular sizes of the hybridizing DNA fragments are given to the left. (B) cpb2 RFLP Southern blot analysis of HpaI-digested DNAs from SD isolates with a 318-bp DIG-labeled cpb2-specific probe. Results are shown for control isolates CWC245 (a cpb2-positive, pig GI disease isolate) and 106527 (a cpb2-negative isolate) and for representative SD isolates 6253/94, 6257/94, 3433/95, and 4178/94. Molecular sizes of the hybridizing DNA fragments are given to the left.