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. 2005 Dec;71(12):8362–8370. doi: 10.1128/AEM.71.12.8362-8370.2005

FIG. 3.

FIG. 3.

PFGE evidence supporting the plasmid localization of cpe and cpb2 in SD isolates. (A) PFGE and Southern blot analysis of undigested (ud) and I-CeuI-digested (d) DNA from each of the C. perfringens isolates specified. Blots were probed with a 639-bp cpe-specific probe. Results are shown for control strains 8239 (NCTC8239; a chromosomal cpe, food poisoning isolate) and F4969 (a plasmid cpe, non-food-borne human GI disease isolate) and for representative SD isolates 7458/94, 6263/94, and 6257/94. The pulsed-field gel was calibrated with bacteriophage lambda DNA markers, whose migrations are shown at the right. (B) PFGE and Southern blot analysis of undigested DNA, prepared in agarose plugs, from each of the C. perfringens isolates specified. Blots were probed with a 318-bp cpb2-specific probe. Results are shown for control strains CWC245 (a cpb2-positive, type C isolate carrying cpb2 on a large plasmid) and for representative SD isolates 6253/94, 6257/94, and 3433/94. The pulsed-field gel was calibrated with bacteriophage lambda DNA markers, whose migrations are shown at the left.